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验证数据展示

  • WB analysis using 84060-5-RR

    WB analysis using 84060-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84060-5-RR (SLC9A3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84060-5-RR

    Affinity and Kinetic Characterization of 84060-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84060-5-RR against Human SLC9A3R2 were performed. The affinity constant is 0.476 nM.

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SLC9A3R2 Recombinant monoclonal antibody

SLC9A3R2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 84060-5-RR

产品说明书

CloneNo. 241215A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

241215A3, E3KARP, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHE3RF2, NHERF 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inT-47D cells, MCF-7 cells, HeLa cells, HEK-293T cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84060-5-RR targets SLC9A3R2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13476

Product name: Recombinant human SLC9A3R2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-337 aa of BC106001

Sequence: MAAPEPLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVNGVNVEGETHHQVVQRIKAVEGQTRLLVVDQETDEELRRRQLTCTEEMAQRGLPPAHDPWEPKPDWAHTGSHSSEAGKKDVSGPLRELRPRLCHLRKGPQGYGFNLHSDKSRPGQYIRSVDPGSPAARSGLRAQDRLIEVNGQNVEGLRHAEVVASIKAREDEARLLVVDPETDEHFKRLRVTPTEEHVEGPLPSPVTNGTSPAQLNGGSACSSRSDLPGSDKDTEDGSAWKQDPFQESGLHLSPTAAEAKEKARAMRVNKRAPQMDWNRKREIFSNF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
别名 241215A3, E3KARP, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHE3RF2, NHERF 2
计算分子量 337 aa, 37 kDa
观测分子量~45 kDa
GenBank蛋白编号BC106001
基因名称 SLC9A3R2
Gene ID (NCBI) 9351
RRIDAB_3671630
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ15599
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC9A3R2, also named as NHERF2, is a member of the Na+/H+ exchanger regulatory factor (NHERF) family of PDZ scaffolding proteins. SLC9A3R2 is needed for cGMP and carbachol-mediated inhibition as well as LPA and dexamethasone-mediated stimulation of NHE3. NHERF1 and NHERF2 are highly homologous structurally to each other but play different roles in the regulation of Na+ and Cl− transport.(PMID: 23612977)

实验方案

Product Specific Protocols
WB protocol for SLC9A3R2 antibody 84060-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols