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验证数据展示

  • IHC staining of rat kidney using 31437-1-AP

    IHC staining of rat kidney using 31437-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31437-1-AP (SLC8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 31437-1-AP

    IHC staining of rat kidney using 31437-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31437-1-AP (SLC8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat cerebellum using 31437-1-AP

    IF Staining of rat cerebellum using 31437-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using SLC8A2 antibody (31437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC8A2 Polyclonal antibody

SLC8A2 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 31437-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IHC, IF-P, ELISA

别名

Sodium/calcium exchanger 2, NCX2, KIAA1087

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat cerebellum tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31437-1-AP targets SLC8A2 in IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag34438

Product name: Recombinant human SLC8A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-68 aa of NM_015063

Sequence: AATPTPSLPPPPANDSDTSTGGCQGSYRCQPGVLLPVWEPDDPSLGDK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 8 (sodium/calcium exchanger), member 2
别名 Sodium/calcium exchanger 2, NCX2, KIAA1087
计算分子量921 aa, 100 kDa
GenBank蛋白编号NM_015063
基因名称 SLC8A2
Gene ID (NCBI) 6543
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UPR5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC8A2, or solute carrier family 8 member 2, belongs to the SLC8 gene family, which encodes sodium-calcium exchangers (NCX). These proteins are part of the CaCA (Ca2+/Cation Antiporter) superfamily and play a significant role in regulating Ca2+-dependent events in many cell types. SLC8A2 has been implicated in various diseases, such as heart failure, arrhythmia, cerebral ischemia, hypertension, diabetes, and muscle dystrophy.

实验方案

Product Specific Protocols
IF protocol for SLC8A2 antibody 31437-1-APDownload protocol
IHC protocol for SLC8A2 antibody 31437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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