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验证数据展示

  • WB analysis of A549 using 84178-5-RR (same clone as 84178-5-PBS)

    WB analysis of A549 using 84178-5-RR (same clone as 84178-5-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 84178-5-RR (SLC7A5 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 84178-5-RR (same clone as 84178-5-PBS)

    IF Staining of HT-29 using 84178-5-RR (same clone as 84178-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using SLC7A5 antibody (84178-5-RR, Clone: 241476D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

  • FC experiment of HT-29 using 84178-5-RR (same clone as 84178-5-PBS)

    FC experiment of HT-29 using 84178-5-RR (same clone as 84178-5-PBS)

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug SLC7A5 Recombinant antibody (84178-5-RR, Clone:241476D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84178-5-RR (same clone as 84178-5-PBS)

    IHC staining of human placenta using 84178-5-RR (same clone as 84178-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84178-5-RR (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC7A5 antibody included
  • IHC staining of human placenta using 84178-5-RR (same clone as 84178-5-PBS)

    IHC staining of human placenta using 84178-5-RR (same clone as 84178-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84178-5-RR (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC7A5 antibody included
  • Affinity and Kinetic Characterization of 84178-5-RR

    Affinity and Kinetic Characterization of 84178-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84178-5-RR against Human SLC7A5 were performed. The affinity constant is 0.319 nM.

SLC7A5 Recombinant antibody, PBS Only

SLC7A5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84178-5-PBS

产品说明书

CloneNo. 241476D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

LAT1, 241476D5, 4F2 LC, 4F2 light chain, 4F2LC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SLC7A5 Ready-To-Use IHC Kit. SLC7A5 primary antibody included.

产品信息

84178-5-PBS targets SLC7A5 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 SLC7A5 fusion protein Ag29164 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
别名 LAT1, 241476D5, 4F2 LC, 4F2 light chain, 4F2LC
计算分子量 507 aa, 55 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC039692
基因名称 SLC7A5
Gene ID (NCBI) 8140
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ01650
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)