验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy SLC7A5 Ready-To-Use IHC Kit. SLC7A5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
67951-1-Ig targets SLC7A5 in WB, IF, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human, goat |
| 免疫原 | SLC7A5 fusion protein Ag20291 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 |
| 别名 | 4F2 LC, 4F2 light chain, 4F2LC, CD98 light chain, CD98LC, D16S469E, E16, hLAT1, Integral membrane protein E16, LAT1, MPE16, SLC7A5 |
| 计算分子量 | 507 aa, 55 kDa |
| 观测分子量 | 35-50 kDa |
| GenBank蛋白编号 | BC039692 |
| 基因名称 | SLC7A5 |
| Gene ID (NCBI) | 8140 |
| RRID | AB_2918703 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q01650 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC7A5 antibody 67951-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Metab Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness | ||
J Med Chem Discovery of Novel Aminobutanoic Acid-Based ASCT2 Inhibitors for the Treatment of Non-Small-Cell Lung Cancer | ||
Anim Nutr Long-term leucine supplementation increases body weight in goats by controlling appetite and muscle protein synthesis under protein-restricted conditions |