验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293 cells, HeLa cells, HepG2 cells, rat brain tissue, mouse kidney tissue, mouse stomach tissue, rat liver tissue |
| Positive IF-P detected in | mouse eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
32384-1-AP targets SLC7A11/xCT in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | SLC7A11/xCT fusion protein Ag38215 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 |
| 别名 | Amino acid transport system xc-, Cystine/glutamate transporter, Slc7a11, Solute carrier family 7 member 11, Subtle gray protein |
| 计算分子量 | 55 kDa |
| 观测分子量 | 70 kDa, 45 kDa |
| GenBank蛋白编号 | NM_011990 |
| 基因名称 | Slc7a11 |
| Gene ID (NCBI) | 26570 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9WTR6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC7A11 (also known as xCT) is a membrane transporter involved in cysteine uptake. It promotes glutathione synthesis through cystine uptake and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers, including lung cancer. Although different from the predicted size of 55 kDa, the 45- and 70-kDa bands represent xCT, most likely a monomer and homodimer of the protein (PMID: 16399997).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC7A11/xCT antibody 32384-1-AP | Download protocol |
| IF protocol for SLC7A11/xCT antibody 32384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
