验证数据展示
经过测试的应用
| Positive WB detected in | unboiled HeLa cells, unboiled mouse brain tissue, unboiled rat brain tissue |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32384-1-AP targets SLC7A11/xCT in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag38215 Product name: Recombinant mouse Slc7a11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-42 aa of NM_011990 Sequence: MVRKPVVATISKGGYLQGNMSGRLPSMGDQEPPGQEKVVLKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 |
| 别名 | Amino acid transport system xc-, Cystine/glutamate transporter, Slc7a11, Solute carrier family 7 member 11, Subtle gray protein |
| 计算分子量 | 55 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | NM_011990 |
| 基因名称 | Slc7a11 |
| Gene ID (NCBI) | 26570 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9WTR6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC7A11 (also known as xCT) is a membrane transporter involved in cysteine uptake. It promotes glutathione synthesis through cystine uptake and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers, including lung cancer. 32384-1-AP recognizes the 35-40 kDa protein similar to the paper published (PMID: 36747082).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for SLC7A11/xCT antibody 32384-1-AP | Download protocol |
| WB protocol for SLC7A11/xCT antibody 32384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep TXNIP mediates ferroptosis in a bronchopulmonary dysplasia mouse model by regulating the SLC7A11/GPX4 pathway | ||
J Orthop Translat MiR-370-3p regulate TLR4/SLC7A11/GPX4 to alleviate the progression of glucocorticoids-induced osteonecrosis of the femoral head by promoting osteogenesis and suppressing ferroptosis. | ||
Ecotoxicol Environ Saf Zearalenone induces intestinal damage and flora disturbance in rats by triggering ferroptosis via the system Xc(-)-GSH-GPX4 signaling pathway. | ||
Sci Rep MiR-125b-5p ameliorates ventilator-induced lung injury in rats by suppressing ferroptosis via the regulation of the Keap1/Nrf2/GPX4 signaling pathway. | ||
J Ethnopharmacol Yishen Qufeng formula mediates ferroptosis to improve renal fibrosis in unilateral ureteral obstruction rats. | ||
Bioorg Med Chem Curcumin derivatives as ferroptosis inducers: design, synthesis and anti breast cancer activity evaluation. |
