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验证数据展示

  • IHC staining of mouse brain using 83412-4-RR

    IHC staining of mouse brain using 83412-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83412-4-RR

    IHC staining of mouse brain using 83412-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 83412-4-RR

    IHC staining of rat brain using 83412-4-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 83412-4-RR

    IHC staining of rat brain using 83412-4-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83412-4-RR (SLC6A7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83412-4-RR

    Affinity and Kinetic Characterization of 83412-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83412-4-RR against Human SLC6A7 were performed. The affinity constant is below 1 pM.

SLC6A7 Recombinant monoclonal antibody

SLC6A7 Uni-rAb® Recombinant Antibody for IHC, ELISA
Cat No. 83412-4-RR

产品说明书

CloneNo. 240395H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

Sodium-dependent proline transporter, PROT, 240395H5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83412-4-RR targets SLC6A7 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34413

Product name: Recombinant human SLC6A7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 138-214 aa of BC093785

Sequence: AYVLFYLFASLTSDLPWEHCGNWWNTELCLEHRVSKDGNGALPLNLTCTVSPSEEYWSRYVLHIQGSQGIGSPGEIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, L-proline), member 7
别名 Sodium-dependent proline transporter, PROT, 240395H5
计算分子量 636 aa, 71 kDa
GenBank蛋白编号BC093785
基因名称 SLC6A7
Gene ID (NCBI) 6534
RRIDAB_3671059
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ99884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC6A7 also known as PROT, belongs to the solute Carrier 6 gene family. SLC6A7 is a high-affinity mammalian brain L-proline transporter, which is different from other sodium-dependent plasma membrane carriers, and has unique pharmacological specificity, kinetic properties, and ionic requirements. SLC6A7 is a brain specific sodium (and chloride)-dependent proline transporter, that terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals(PMID: 7651355).

实验方案

Product Specific Protocols
IHC protocol for SLC6A7 antibody 83412-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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