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验证数据展示

  • WB analysis using 30843-1-AP (same clone as 30843-1-PBS)

    WB analysis using 30843-1-AP (same clone as 30843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30843-1-AP (SLC6A20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30843-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 30843-1-AP (same clone as 30843-1-PBS)

    IF Staining of mouse brain using 30843-1-AP (same clone as 30843-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC6A20 antibody (30843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30843-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 30843-1-AP (same clone as 30843-1-PBS)

    IF Staining of mouse brain using 30843-1-AP (same clone as 30843-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC6A20 antibody (30843-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30843-1-PBS in a different storage buffer formulation.

SLC6A20 Polyclonal antibody, PBS Only

SLC6A20 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 30843-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, Indirect ELISA

别名

SIT1, Sodium- and chloride-dependent transporter XTRP3, Sodium/imino-acid transporter 1, XT3, XTRP3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30843-1-PBS targets SLC6A20 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag33967

Product name: Recombinant human SLC6A20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 298-389 aa of BC136431

Sequence: YGFKATFNYENCLKKVSLLLTNTFDLEDGFLTASNLEQVKGYLASAYPSKYSEMFPQIKNCSLESELDTAVQGTGLAFIVYTEAIKNMEVSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 6 (proline IMINO transporter), member 20
别名 SIT1, Sodium- and chloride-dependent transporter XTRP3, Sodium/imino-acid transporter 1, XT3, XTRP3
观测分子量 50-60 kDa
GenBank蛋白编号BC136431
基因名称 SLC6A20
Gene ID (NCBI) 54716
RRIDAB_3669764
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NP91
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC6A20 also known as XT3, SIT1, belongs to the family of solute carrier 6, mediating the transport of neutral amino acids from the extracellular milieu toward cell or storage vesicles utilizing an electric membrane potential as the driving force. SLC6A20 is expressed in the kidney and small intestine and is localized in the brush marginal membrane of the proximal renal tubules (PMID: 16174864). Mutations in SLC6A20 are associated with Hyperglycinuria and Iminoglycinuria (PMID: 19033659)

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