验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| FC | See 1 publications below |
产品信息
28488-1-AP targets GAT1 in WB, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, FC |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag29712 Product name: Recombinant human SLC6A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-52 aa of BC033904 Sequence: MATNGSKVADGQISTEVSEAPVANDKPKTLVVKVQKKAADLPDRDTWKGRFD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, GABA), member 1 |
| 别名 | GABATHG, GABATR, GABT1, GAT 1, GAT1, SLC6A1 |
| 计算分子量 | 67 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC033904 |
| 基因名称 | GABA Transporter 1/GAT1 |
| Gene ID (NCBI) | 6529 |
| RRID | AB_2881154 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30531 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GAT1 antibody 28488-1-AP | Download protocol |
| WB protocol for GAT1 antibody 28488-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model. | ||
Neuropharmacology A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine | ||
Cell Biosci Amelioration of olfactory dysfunction in a mouse model of Parkinson's disease via enhancing GABAergic signaling | ||
J Exp Clin Cancer Res GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway |
