验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
28488-1-AP targets GAT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag29712 Product name: Recombinant human SLC6A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-52 aa of BC033904 Sequence: MATNGSKVADGQISTEVSEAPVANDKPKTLVVKVQKKAADLPDRDTWKGRFD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, GABA), member 1 |
| 别名 | SLC6A1, GABA Transporter 1, GABATHG, GABATR, GABT1 |
| 计算分子量 | 67 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC033904 |
| 基因名称 | GABA Transporter 1/GAT1 |
| Gene ID (NCBI) | 6529 |
| RRID | AB_2881154 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30531 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAT1 (GABA Transporter 1) is a vital membrane transporter in the central nervous system, which is mainly responsible for clearing the inhibitory neurotransmitter-GABA in synaptic cleft. GAT1 belongs to Solute Carrier Family 6(SLC6), and GABA in synaptic cleft is reabsorbed into presynaptic neurons or astrocytes by using the gradient of sodium ion (Na) and chloride ion (Cl). It is a key regulator to regulate inhibitory signal transduction in nervous system. By limiting the existence time of GABA in synaptic cleft, GAT1 directly controls the intensity and duration of inhibitory nerve transmission. GAT1 is a famous target of antiepileptic drugs. For example, Tiagabine is an efficient GAT1 inhibitor, which can block GAT1 function and increase the concentration of GABA in synaptic cleft, thus enhancing the inhibition and treating epilepsy.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for GAT1 antibody 28488-1-AP | Download protocol |
| WB protocol for GAT1 antibody 28488-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model. | ||
Neuropharmacology A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine | ||
Cell Biosci Amelioration of olfactory dysfunction in a mouse model of Parkinson's disease via enhancing GABAergic signaling | ||
J Exp Clin Cancer Res GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway | ||
J Neurosci Identification of New Ciliary Signaling Pathways in the Brain and Insights into Neurological Disorders |
