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SLC44A1 Polyclonal antibody

SLC44A1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14687-1-AP

Print datasheet

Synonyms

CD92, CDW92, choline transporter-like protein 1, CHTL1, CTL1, SLC44A1

验证数据展示

  • WB analysis using 14687-1-AP

    WB analysis using 14687-1-AP

    HeLa lysates (heated at 100℃ and 37℃) were subjected to SDS PAGE followed by western blot with 14687-1-AP (SLC44A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 14687-1-AP

    IHC staining of human lung cancer using 14687-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14687-1-AP (SLC44A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected in37°C incubated HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14687-1-AP targets SLC44A1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SLC44A1 fusion protein Ag6024 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 44, member 1
Synonyms CD92, CDW92, choline transporter-like protein 1, CHTL1, CTL1, SLC44A1
Calculated Molecular Weight 73 kDa
Observed Molecular Weight70-73 kDa
GenBank Accession NumberBC049203
Gene Symbol SLC44A1
Gene ID (NCBI) 23446
RRIDAB_10640573
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWI5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC44A1, also referred to as choline transporter-like protein 1 (CTL1) or CDw92, is a choline transporter detected in both the plasma and mitochondrial membranes where it transports choline at high affinity and in a Na+-independent manner.

实验方案

Product Specific Protocols
WB protocol for SLC44A1 antibody 14687-1-APDownload protocol
IHC protocol for SLC44A1 antibody 14687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Exp Med

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Authors - Yalong Wang
View Article
mouseIHC

Cell Prolif

The nano-windmill exerts superior anti-inflammatory effects via reducing choline uptake to inhibit macrophage activation

Authors - Nanxin Liu
View Article
humanIHC

BMC Cancer

Novel biomarkers to predict treatment response and prognosis in locally advanced rectal cancer undergoing neoadjuvant chemoradiotherapy

Authors - Bingjie Guan
View Article
mouseIF

Cell Metab

Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.

Authors - Elsa Sanchez-Lopez
View Article