验证数据展示
经过测试的应用
| Positive WB detected in | 37°C incubated HeLa cells, HEK-293 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14687-1-AP targets SLC44A1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6024 Product name: Recombinant human SLC44A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 55-208 aa of BC049203 Sequence: AARLVSGYDSYGNICGQKNTKLEAIPNSGMDHTQRKYVFFLDPCNLDLINRKIKSVALCVAACPRQELKTLSDVQKFAEINGSALCSYNLKPSEYTTSPKSSVLCPKLPVPASAPIPFFHRCAPVNISCYAKFAEALITFVSDNSVLHRLISGV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 44, member 1 |
| 别名 | CTL1, CHTL1, Choline transporter-like protein 1, CDw92, CD92 |
| 计算分子量 | 73 kDa |
| 观测分子量 | 70-73 kDa |
| GenBank蛋白编号 | BC049203 |
| 基因名称 | SLC44A1 |
| Gene ID (NCBI) | 23446 |
| RRID | AB_10640573 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WWI5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC44A1, also referred to as choline transporter-like protein 1 (CTL1) or CDw92, is a choline transporter detected in both the plasma and mitochondrial membranes where it transports choline at high affinity and in a Na+-independent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC44A1 antibody 14687-1-AP | Download protocol |
| IHC protocol for SLC44A1 antibody 14687-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
Cell Prolif The nano-windmill exerts superior anti-inflammatory effects via reducing choline uptake to inhibit macrophage activation | ||
BMC Cancer Novel biomarkers to predict treatment response and prognosis in locally advanced rectal cancer undergoing neoadjuvant chemoradiotherapy | ||
Cell Metab Transketolase promotes MAFLD by limiting inosine-induced mitochondrial activity | ||
