验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19429-1-AP targets ZIP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ZIP7 fusion protein Ag13762 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 39 (zinc transporter), member 7 |
| 别名 | KE4, RING5, SLC39A7, Zinc transporter SLC39A7, ZIP 7, ZIP7 |
| 计算分子量 | 469 aa, 50 kDa |
| 观测分子量 | 45-50 kDa, 56 kDa |
| GenBank蛋白编号 | BC000645 |
| 基因名称 | ZIP7 |
| Gene ID (NCBI) | 7922 |
| RRID | AB_10643376 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92504 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZIP7 is a functional zinc transporter transporting zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 is post-translationally regulated by CK2-mediated phosphorylation. This ZIP7 phosphorylation results in zinc release from intracellular stores, which activates multiple tyrosine kinases and regulate cell survival and proliferation. Dual bands of 50 kDa and 56 kDa detected by this antibody may represent the native and phosphorylated forms of ZIP7, respectively. (PMID: 28232492, 28205653)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| IHC protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| IF protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| IP protocol for ZIP7 antibody 19429-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Chem Sci X-ray fluorescence imaging reveals subcellular biometal disturbances in a childhood neurodegenerative disorder. | ||
Cell Death Differ Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. | ||
Acta Neuropathol Commun Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.
| ||
Metallomics Deregulation of biometal homeostasis: the missing link for neuronal ceroid lipofuscinoses? | ||
