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验证数据展示

  • WB analysis of Caco-2 using 83346-6-RR (same clone as 83346-6-PBS)

    WB analysis of Caco-2 using 83346-6-RR (same clone as 83346-6-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83346-6-PBS in a different storage buffer formulation.

  • WB analysis of human liver using 83346-6-RR (same clone as 83346-6-PBS)

    WB analysis of human liver using 83346-6-RR (same clone as 83346-6-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83346-6-PBS in a different storage buffer formulation.

  • FC experiment of Caco-2 using 83346-6-RR (same clone as 83346-6-PBS)

    FC experiment of Caco-2 using 83346-6-RR (same clone as 83346-6-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83346-6-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83346-6-RR (same clone as 83346-6-PBS)

    FC experiment of U-251 using 83346-6-RR (same clone as 83346-6-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83346-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83346-6-RR

    Affinity and Kinetic Characterization of 83346-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83346-6-RR against Human SLC38A7 were performed. The affinity constant is 1.31 nM.

    {{ptg:ProductFeaturesInfo}}

SLC38A7 Recombinant monoclonal antibody, PBS Only

SLC38A7 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83346-6-PBS

产品说明书

CloneNo. 240147C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83346-6-PBS targets SLC38A7 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35081

Product name: Recombinant human SLC38A7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-53 aa of BC001961

Sequence: MAQVSINNDYSEWDLSTDAGERARLLQSPCVDTAPKSEWEASPGGLDRGTTST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 38, member 7
别名 SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815
计算分子量 50 kDa
观测分子量 37 kDa
GenBank蛋白编号BC001961
基因名称 SLC38A7
Gene ID (NCBI) 55238
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NVC3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC38A7 also known as SNAT7, is a member of the SLC38 family that encodes sodium-coupled neutral amino acid transporters. SLC38A7 is a system N transporter that has a substrate preference for L-glutamine. It also transports other amino acids with polar side chains, as well as L-histidine and L-alanine (PMID: 21511949).