验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, mouse kidney tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
20612-1-AP targets SLC37A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14660 Product name: Recombinant human SLC37A4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-93 aa of BC015650 Sequence: MAAQGYGYYRTVIFSAMFGGYSLYYFNRKTFSFVMPSLVEEIPLDKDDLGFITSSQSAAYAISKFVSGVLSDQMSARWLFSSGLLLVGLVNIF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 37 (glucose-6-phosphate transporter), member 4 |
| 别名 | Glucose-6-phosphate exchanger SLC37A4, Glucose-5-phosphate transporter, G6PT3, G6PT2, G6PT1 |
| 计算分子量 | 429 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC015650 |
| 基因名称 | SLC37A4 |
| Gene ID (NCBI) | 2542 |
| RRID | AB_10858386 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43826 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC37A4 antibody 20612-1-AP | Download protocol |
| WB protocol for SLC37A4 antibody 20612-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Discov Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p | ||
Sci Rep SLC37A4, gene responsible for glycogen storage disease type 1b, regulates gingival epithelial barrier function via JAM1 expression | ||
J Adv Res SRSF9 mediates oncogenic RNA splicing of SLC37A4 via liquid-liquid phase separation to promote oral cancer progression |
