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验证数据展示

  • WB analysis of mouse testis using 67929-1-Ig

    WB analysis of mouse testis using 67929-1-Ig

    unboiled mouse testis tissue were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 67929-1-Ig

    IHC staining of mouse testis using 67929-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67929-1-Ig (SLC36A3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 67929-1-Ig

    IF Staining of mouse testis using 67929-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SLC36A3 antibody (67929-1-Ig, Clone: 1A8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse testis using 67929-1-Ig

    IF Staining of mouse testis using 67929-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SLC36A3 antibody (67929-1-Ig, Clone: 1A8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SLC36A3 Monoclonal antibody

SLC36A3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67929-1-Ig

产品说明书

CloneNo. 1A8E6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, IHC, IF-P, ELISA

别名

FLJ16658, PAT3, SLC36A3, TRAMD2, Tramdorin 2, tramdorin2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled mouse testis tissue, COLO 320 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67929-1-Ig targets SLC36A3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 SLC36A3 fusion protein Ag16556 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 36 (proton/amino acid symporter), member 3
别名 FLJ16658, PAT3, SLC36A3, TRAMD2, Tramdorin 2, tramdorin2
计算分子量 511 aa, 56 kDa
观测分子量51 kDa
GenBank蛋白编号BC101092
基因名称 SLC36A3
Gene ID (NCBI) 285641
RRIDAB_2918681
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ495N2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC36A3 antibody 67929-1-IgDownload protocol
IHC protocol for SLC36A3 antibody 67929-1-IgDownload protocol
IF protocol for SLC36A3 antibody 67929-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols