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验证数据展示

  • IF Staining of U2OS using 83085-3-RR

    IF Staining of U2OS using 83085-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC35F4 antibody (83085-3-RR, Clone: 230360B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of U2OS using 83085-3-RR

    FC experiment of U2OS using 83085-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SLC35F4 (83085-3-RR, Clone:230360B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83085-3-RR

    Affinity and Kinetic Characterization of 83085-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83085-3-RR against Human SLC35F4 were performed. The affinity constant is 0.92 nM.

SLC35F4 Recombinant antibody

SLC35F4 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83085-3-RR

产品说明书

CloneNo. 230360B7

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IF/ICC, FC (Intra), ELISA

别名

C14orf36, 230360B7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83085-3-RR targets SLC35F4 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 Human
免疫原 SLC35F4 fusion protein Ag34518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 35, member F4
别名 C14orf36, 230360B7
计算分子量521 aa, 58 kDa
GenBank蛋白编号NM_001206920
基因名称 SLC35F4
Gene ID (NCBI) 341880
RRIDAB_3670801
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SLC35F4 antibody 83085-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols