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验证数据展示

  • WB analysis using 82895-2-RR

    WB analysis using 82895-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82895-2-RR (SLC30A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 82895-2-RR

    FC experiment of U2OS using 82895-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SLC30A1 Recombinant antibody (82895-2-RR, Clone:230152E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 82895-2-RR

    Affinity and Kinetic Characterization of 82895-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82895-2-RR against Human SLC30A1 were performed. The affinity constant is 1.49 nM.

SLC30A1 Recombinant monoclonal antibody

SLC30A1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82895-2-RR

产品说明书

CloneNo. 230152E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

230152E10, Zinc transporter 1, ZNT1, ZnT-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSW480 cells, HeLa cells, HepG2 cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82895-2-RR targets SLC30A1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18567

Product name: Recombinant human SLC30A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 330-507 aa of BC121015

Sequence: YTTYPLLKESALILLQTVPKQIDIRNLIKELRNVEGVEEVHELHVWQLAGSRIIATAHIKCEDPTSYMEVAKTIKDVFHNHGIHATTIQPEFASVGSKSSVVPCELACRTQCALKQCCGTLPQAPSGKDAEKTPAVSISCLELSNNLEKKPRRTKAENIPAVVIEIKNMPNKQPESSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 30 (zinc transporter), member 1
别名 230152E10, Zinc transporter 1, ZNT1, ZnT-1
计算分子量 507 aa, 55 kDa
观测分子量63~75 kDa
GenBank蛋白编号BC121015
基因名称 SLC30A1
Gene ID (NCBI) 7779
RRIDAB_3670624
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6M5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). Western analysis detected a higher band at ~63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319).

实验方案

Product Specific Protocols
WB protocol for SLC30A1 antibody 82895-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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