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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82895-2-RR (same clone as 82895-2-PBS)

    WB analysis using 82895-2-RR (same clone as 82895-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82895-2-RR (SLC30A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82895-2-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 82895-2-RR (same clone as 82895-2-PBS)

    FC experiment of U2OS using 82895-2-RR (same clone as 82895-2-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SLC30A1 Recombinant antibody (82895-2-RR, Clone:230152E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82895-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82895-2-RR

    Affinity and Kinetic Characterization of 82895-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82895-2-RR against Human SLC30A1 were performed. The affinity constant is 1.49 nM.

SLC30A1 Recombinant antibody, PBS Only

SLC30A1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82895-2-PBS

产品说明书

CloneNo. 230152E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

ZnT-1, ZNT1, Zinc transporter 1, 230152E10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82895-2-PBS targets SLC30A1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 SLC30A1 fusion protein Ag18567 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 30 (zinc transporter), member 1
别名 ZnT-1, ZNT1, Zinc transporter 1, 230152E10
计算分子量 507 aa, 55 kDa
观测分子量63~75 kDa
GenBank蛋白编号BC121015
基因名称 SLC30A1
Gene ID (NCBI) 7779
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6M5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). Western analysis detected a higher band at ~63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319).