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验证数据展示

  • WB analysis using 18182-1-AP

    WB analysis using 18182-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 18182-1-AP

    IHC staining of human pancreas cancer using 18182-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 18182-1-AP

    IHC staining of human pancreas cancer using 18182-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 18182-1-AP

    IF Staining of HepG2 using 18182-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SLC28A3 antibody (18182-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SLC28A3 Polyclonal antibody

SLC28A3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18182-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

hCNT3, Concentrative Na(+)-nucleoside cotransporter 3, CNT3, CNT 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18182-1-AP targets SLC28A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12887

Product name: Recombinant human SLC28A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-186 aa of BC093821

Sequence: MELRSTAAPRAEGYSNVGFQNEENFLENENTSGNNSIRSRAVQSREHTNTKQDEEQVTVEQDSPRNREHMEDDDEEMQQKGCLERRYDTVCGFCRKHKTTLRHIIWGILLAGYLVMVISACVLNFHRALPLFVITVAAIFFVVWDHLMAKYEHRIDEMLSPGRRLLNSHWFWLKWVIWSSLVLAVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 28 (sodium-coupled nucleoside transporter), member 3
别名 hCNT3, Concentrative Na(+)-nucleoside cotransporter 3, CNT3, CNT 3
计算分子量 691 aa, 77 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC093821
基因名称 SLC28A3
Gene ID (NCBI) 64078
RRIDAB_3085558
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAS3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC28A3 (Solute carrier family 28 member 3), also named CNT3, has sodium-dependent, pyrimidine- and purine-selective (PMID: 11032837, PMID: 15861042, PMID: 16446384, PMID: 17140564, PMID: 21998139). It is involved in the homeostasis of endogenous nucleosides (PMID: 11032837, PMID: 15861042). SLC28A3 is able to transports uridine, gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (PMID: 21795683, PMID: 11032837, PMID: 17140564). SLC28A3, the human concentrative nucleoside transporter, plays a role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs (PMID: 15738947). Variants in SLC28A3 and UGT1A6 act as genetic markers predictive of anthracycline-induced cardiotoxicity (PMID: 23441093).

实验方案

Product Specific Protocols
IF protocol for SLC28A3 antibody 18182-1-APDownload protocol
IHC protocol for SLC28A3 antibody 18182-1-APDownload protocol
WB protocol for SLC28A3 antibody 18182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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