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验证数据展示

  • WB analysis of A549 using 22726-1-AP (same clone as 22726-1-PBS)

    WB analysis of A549 using 22726-1-AP (same clone as 22726-1-PBS)

    37°C incubated A549 cells were subjected to SDS PAGE followed by western blot with 22726-1-AP (SLC26A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22726-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 22726-1-AP (same clone as 22726-1-PBS)

    IHC staining of mouse kidney using 22726-1-AP (same clone as 22726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22726-1-AP (SLC26A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22726-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 22726-1-AP (same clone as 22726-1-PBS)

    IHC staining of mouse lung using 22726-1-AP (same clone as 22726-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22726-1-AP (SLC26A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22726-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 22726-1-AP (same clone as 22726-1-PBS)

    IP experiment of mouse kidney using 22726-1-AP (same clone as 22726-1-PBS)

    IP result of anti-SLC26A4 (IP:22726-1-AP, 4ug; Detection:22726-1-AP 1:500) with mouse kidney tissue lysate 4800ug. This data was developed using the same antibody clone with 22726-1-PBS in a different storage buffer formulation.

SLC26A4 Polyclonal antibody, PBS Only

SLC26A4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 22726-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

DFNB4, EVA, PDS, Pendrin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

22726-1-PBS targets SLC26A4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18622

Product name: Recombinant human SLC26A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 517-646 aa of BC153002

Sequence: SWNGLGSIPSTDIYKSTKNYKNIEEPQGVKILRFSSPIFYGNVDGFKKCIKSTVGFDAIRVYNKRLKALRKIQKLIKSGQLRATKNGIISDAVSTNNAFEPDEDIEDLEELDIPTKEIEIQVDWNSELPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 26, member 4
别名 DFNB4, EVA, PDS, Pendrin
计算分子量 780 aa, 86 kDa
观测分子量 90 kDa
GenBank蛋白编号BC153002
基因名称 SLC26A4
Gene ID (NCBI) 5172
RRIDAB_3742079
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43511
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC26A4 encodes an 86-kDa transmembrane protein named pendrin with up to 15 predicted membrane-spanning domains (PMID: 22116369). SLC26A4 is necessary for normal reabsorption of endolymph, the fluid bathing the inner ear. (PMID: 31599023, PMID: 39383236). In the endolymphatic sac, where fluid reabsorption occurs, SLC26A4 is localized along the apical microvilli of mitochondria-rich cells, in contact with the endolymph, and associated with clathrin-coated pits where μ2 and AP-2 are present (PMID: 39383236). SLC26A4 encodes an anion transporter transmembrane protein, pendrin, which is expressed in the thyroid, kidney, and cochlea (PMID: 23185506).

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