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验证数据展示

  • WB analysis of A549 using 22726-1-AP

    WB analysis of A549 using 22726-1-AP

    37°C incubated A549 cells were subjected to SDS PAGE followed by western blot with 22726-1-AP (SLC26A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 22726-1-AP

    IHC staining of mouse kidney using 22726-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22726-1-AP (SLC26A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 22726-1-AP

    IHC staining of mouse lung using 22726-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22726-1-AP (SLC26A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse kidney using 22726-1-AP

    IP experiment of mouse kidney using 22726-1-AP

    IP result of anti-SLC26A4 (IP:22726-1-AP, 4ug; Detection:22726-1-AP 1:500) with mouse kidney tissue lysate 4800ug.

SLC26A4 Polyclonal antibody

SLC26A4 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 22726-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

DFNB4, EVA, PDS, Pendrin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in37°C incubated A549 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse kidney tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22726-1-AP targets SLC26A4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18622

Product name: Recombinant human SLC26A4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 517-646 aa of BC153002

Sequence: SWNGLGSIPSTDIYKSTKNYKNIEEPQGVKILRFSSPIFYGNVDGFKKCIKSTVGFDAIRVYNKRLKALRKIQKLIKSGQLRATKNGIISDAVSTNNAFEPDEDIEDLEELDIPTKEIEIQVDWNSELPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 26, member 4
别名 DFNB4, EVA, PDS, Pendrin
计算分子量 780 aa, 86 kDa
观测分子量 90 kDa
GenBank蛋白编号BC153002
基因名称 SLC26A4
Gene ID (NCBI) 5172
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43511
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SLC26A4 antibody 22726-1-APDownload protocol
IP protocol for SLC26A4 antibody 22726-1-APDownload protocol
WB protocol for SLC26A4 antibody 22726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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