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验证数据展示

  • WB analysis of mouse brain using 27869-1-AP

    WB analysis of mouse brain using 27869-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27869-1-AP (SLC25A46 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27869-1-AP

    IHC staining of human colon using 27869-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27869-1-AP (SLC25A46 antibody) at dilution of 1:200 (under 10x lens).

  • IF Staining of NIH/3T3 using 27869-1-AP

    IF Staining of NIH/3T3 using 27869-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 27869-1-AP (SLC25A46 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SLC25A46 Polyclonal antibody

SLC25A46 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27869-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

SLC25A46, TB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman colon tissue, human medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

This antibody is only recommended for WB detection of tissue samples.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27869-1-AP targets SLC25A46 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 SLC25A46 fusion protein Ag27293 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25, member 46
别名 SLC25A46, TB1
计算分子量 418 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC017169
基因名称 SLC25A46
Gene ID (NCBI) 91137
RRIDAB_2880999
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96AG3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Solute carrier family 25 member 46 (SLC25A46), a highly conserved protein among various species including vertebrates, is a nuclear gene encoding mitochondrial carriers. SLC25A46 is involved in mitochondrial fission and the transport of lipids from the endoplasmic reticulum to mitochondria (PMID: 33277645). SLC25A46 has 3 isoforms with molecular weights of 30, 37 and 46 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for SLC25A46 antibody 27869-1-APDownload protocol
IHC protocol for SLC25A46 antibody 27869-1-APDownload protocol
IF protocol for SLC25A46 antibody 27869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols