验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, HT-29 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
86163-1-RR targets SLC25A22 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | SLC25A22 fusion protein Ag19958 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | solute carrier family 25 (mitochondrial carrier: glutamate), member 22 |
别名 | GC 1, GC1, GC-1, Glutamate/H(+) symporter 1, NET44 |
计算分子量 | 323 aa, 34 kDa |
观测分子量 | 30-34 kDa |
GenBank蛋白编号 | BC023545 |
基因名称 | SLC25A22 |
Gene ID (NCBI) | 79751 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9H936 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)
实验方案
Product Specific Protocols | |
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WB protocol for SLC25A22 antibody 86163-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |