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验证数据展示

  • IF Staining of COS-7 using 25402-1-AP (same clone as 25402-1-PBS)

    IF Staining of COS-7 using 25402-1-AP (same clone as 25402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COS-7 cells using 25402-1-AP (SLC25A22 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

    WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

    IHC staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25402-1-AP (SLC25A22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

    WB analysis using 25402-1-AP (same clone as 25402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25402-1-AP (SLC25A22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

    IF Staining of mouse brain using 25402-1-AP (same clone as 25402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLC25A22 antibody (25402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25402-1-PBS in a different storage buffer formulation.

SLC25A22 Polyclonal antibody, PBS Only

SLC25A22 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 25402-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25402-1-PBS targets SLC25A22 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag19958

Product name: Recombinant human SLC25A22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 128-188 aa of BC023545

Sequence: KIQLQDAGRIAAQRKILAAQGQLSAQGGAQPSVEAPAAPRPTATQLTRDLLRSRGIAGLYK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier: glutamate), member 22
别名
计算分子量 323 aa, 34 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC023545
基因名称 SLC25A22
Gene ID (NCBI) 79751
RRIDAB_2880060
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H936
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)

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