验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, Caco-2 cells, HT-29 cells, rat brain tissue, NCI-H1299 cells |
| Positive IHC detected in | mouse brain tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | COS-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
25402-1-AP targets SLC25A22 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag19958 Product name: Recombinant human SLC25A22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 128-188 aa of BC023545 Sequence: KIQLQDAGRIAAQRKILAAQGQLSAQGGAQPSVEAPAAPRPTATQLTRDLLRSRGIAGLYK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 25 (mitochondrial carrier: glutamate), member 22 |
| 别名 | NET44, Glutamate/H(+) symporter 1, GC-1, GC1, GC 1 |
| 计算分子量 | 323 aa, 34 kDa |
| 观测分子量 | 30-34 kDa |
| GenBank蛋白编号 | BC023545 |
| 基因名称 | SLC25A22 |
| Gene ID (NCBI) | 79751 |
| RRID | AB_2880060 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H936 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SLC25A22 antibody 25402-1-AP | Download protocol |
| IHC protocol for SLC25A22 antibody 25402-1-AP | Download protocol |
| WB protocol for SLC25A22 antibody 25402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Dual-process brain mitochondria isolation preserves function and clarifies protein composition. | ||
Cancer Discov Copper Drives Remodeling of Metabolic State and Progression of Clear Cell Renal Cell Carcinoma |
