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验证数据展示

  • WB analysis of HeLa using 15317-1-AP (same clone as 15317-1-PBS)

    WB analysis of HeLa using 15317-1-AP (same clone as 15317-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

    IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

    IHC staining of human liver cancer using 15317-1-AP (same clone as 15317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 15317-1-AP (same clone as 15317-1-PBS)

    IF Staining of MCF-7 using 15317-1-AP (same clone as 15317-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody (15317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15317-1-PBS in a different storage buffer formulation.

SLC25A15 Polyclonal antibody, PBS Only

SLC25A15 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15317-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15317-1-PBS targets SLC25A15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7563

Product name: Recombinant human SLC25A15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC002702

Sequence: MKSNPAIQAAIDLTAGAAGGTACVLTGQPFDTMKVKMQTFPDLYRGLTDCCLKTYSQVGFRGFYKGTSPALIANIAENSVLFMCYGFCQQVVRKVAGLDKQAKLSDLQNAAAGSFASAFAALVLCPTELVKCRLQTMYEMETSGKIAKSQNTVWSVIKSILRKDGPLGFYHGLSSTLLREVPGYFFFFGGYELSRSFFASGRSKDELGPVPLMLSGGVGGICLWLAVYPVDCIKSRIQVLSMSGKQAGFIRTFLNVVKNEGITALYSGLKPTMIRAFPANGALFLAYEYSRKLMMNQLEAY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
别名
计算分子量 33 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC002702
基因名称 SLC25A15
Gene ID (NCBI) 10166
RRIDAB_2189758
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y619
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC25A15 gene encodes a 301-amino-acid-long mitochondrial carrier protein termed ORC1., which catalyzes the transport of cytosolic ornithine into mitochondria in exchange for citrulline (PMID: 19242930). Some mutations in the SLC25A15 gene have been reported to break the ornithine cycle and be related with human hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome (PMID: 19242930, 24721342). This antibody detected SLC25A15 at an apprarant molecular mass about 33 kDa.

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