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验证数据展示

  • WB analysis of HeLa using 15317-1-AP

    WB analysis of HeLa using 15317-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15317-1-AP (SLC25A15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15317-1-AP

    IHC staining of human liver cancer using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 15317-1-AP

    IHC staining of human liver cancer using 15317-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15317-1-AP (SLC25A15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 15317-1-AP

    IF Staining of MCF-7 using 15317-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody (15317-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SLC25A15 Polyclonal antibody

SLC25A15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15317-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ORNT1, ORC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, mouse stomach tissue
Positive IHC detected inhuman liver cancer tissue, human skin tissue, human brain tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15317-1-AP targets SLC25A15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC25A15 fusion protein Ag7563 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15
别名 ORNT1, ORC1
计算分子量 33 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC002702
基因名称 SLC25A15
Gene ID (NCBI) 10166
RRIDAB_2189758
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y619
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A15 gene encodes a 301-amino-acid-long mitochondrial carrier protein termed ORC1., which catalyzes the transport of cytosolic ornithine into mitochondria in exchange for citrulline (PMID: 19242930). Some mutations in the SLC25A15 gene have been reported to break the ornithine cycle and be related with human hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome (PMID: 19242930, 24721342). This antibody detected SLC25A15 at an apprarant molecular mass about 33 kDa.

实验方案

Product Specific Protocols
WB protocol for SLC25A15 antibody 15317-1-APDownload protocol
IHC protocol for SLC25A15 antibody 15317-1-APDownload protocol
IF protocol for SLC25A15 antibody 15317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols