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验证数据展示

  • IHC staining of human breast cancer using 26804-1-AP

    IHC staining of human breast cancer using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26804-1-AP

    IHC staining of human breast cancer using 26804-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 26804-1-AP

    WB analysis using 26804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse heart using 26804-1-AP

    IF Staining of mouse heart using 26804-1-AP

    Immunofluorescent analysis of (4% PFA) fixed tissue using 26804-1-AP (SLC25A12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SLC25A12 Polyclonal antibody

SLC25A12 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 26804-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26804-1-AP targets SLC25A12 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC25A12 fusion protein Ag25150 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier, Aralar), member 12
别名 AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial
计算分子量 75 kDa, 63 kDa
观测分子量~70 kDa
GenBank蛋白编号BC016932
基因名称 SLC25A12
Gene ID (NCBI) 8604
RRIDAB_2880641
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75746
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).

实验方案

Product Specific Protocols
WB protocol for SLC25A12 antibody 26804-1-APDownload protocol
IHC protocol for SLC25A12 antibody 26804-1-APDownload protocol
IF protocol for SLC25A12 antibody 26804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols