验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27591-1-AP targets SLC24A3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag26297 Product name: Recombinant human SLC24A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-450 aa of NM_020689 Sequence: MSRAYTNGESEVAIKIPIKHTVENGTGPSSAPDRGVNGTRRDDVVAEAGNETENENEDNENDEEEEEDEDDDEGPYTPFDTPSGKLETVKW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 24 (sodium/potassium/calcium exchanger), member 3 |
| 别名 | NCKX3 |
| 计算分子量 | 72 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | NM_020689 |
| 基因名称 | SLC24A3 |
| Gene ID (NCBI) | 57419 |
| RRID | AB_3742241 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HC58 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC24A3, also known as NCKX3 (Na⁺/K⁺/Ca²⁺ exchanger 3), is a member of the solute carrier family 24 (SLC24), which comprises five K⁺-dependent Na⁺/Ca²⁺ exchangers (NCKX1-5). These transporters are critical for maintaining intracellular calcium homeostasis by coupling the extrusion of one Ca²⁺ ion and one K⁺ ion to the influx of four Na⁺ ions. SLC24A3 plays a key role in calcium signaling and homeostasis, influencing processes such as neurotransmission, smooth muscle contraction, and female reproductive physiology. Relatively high levels of SLC24A3 expression can be observed in heart tissue, consistent with the expression patterns reported in the literature. The observed molecular weight of SLC24A3 is 50-60 kDa, which is consistent with the literature description. (PMID: 21573014, PMID: 28588505)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC24A3 antibody 27591-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
