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验证数据展示

  • IHC staining of mouse brain using 24901-1-AP

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24901-1-AP

    IHC staining of mouse brain using 24901-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24901-1-AP (SLC22A23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of L02 using 24901-1-AP

    WB analysis of L02 using 24901-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24901-1-AP (SLC22A23 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 24901-1-AP

    IP experiment of mouse liver using 24901-1-AP

    IP result of anti-SLC22A23 (IP:24901-1-AP, 4ug; Detection:24901-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

SLC22A23 Polyclonal antibody

SLC22A23 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24901-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

BOCT2, C6orf85, SLC22A23

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24901-1-AP targets SLC22A23 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19036

Product name: Recombinant human SLC22A23 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 84-186 aa of BC128580

Sequence: ESLRWLMATQQFESAKRLILHFTQKNRMNPEGDIKGVIPELEKELSRRPKKVCIVKVVGTRNLWKNIVVLCVNSLTGYGIHHCFARSMMGHEVKVPLLENFYA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 22, member 23
别名 BOCT2, C6orf85, SLC22A23
计算分子量 686 aa, 74 kDa
观测分子量 66-74 kDa
GenBank蛋白编号BC128580
基因名称 SLC22A23
Gene ID (NCBI) 63027
RRIDAB_2879787
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA1A5C7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SLC22A23 antibody 24901-1-APDownload protocol
IP protocol for SLC22A23 antibody 24901-1-APDownload protocol
WB protocol for SLC22A23 antibody 24901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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