验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, HEK-293 cells, K-562 cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20355-1-AP targets MCT2/SLC16A7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MCT2/SLC16A7 fusion protein Ag14201 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 16, member 7 (monocarboxylic acid transporter 2) |
| 别名 | SLC16A7, Solute carrier family 16 member 7, Monocarboxylate transporter 2, MCT2, MCT 2 |
| 计算分子量 | 478 aa, 52 kDa |
| 观测分子量 | 40-52 kDa |
| GenBank蛋白编号 | BC030693 |
| 基因名称 | MCT2 |
| Gene ID (NCBI) | 9194 |
| RRID | AB_2878680 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60669 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC16A7, also named MCT2, is a proton-coupled monocarboxylate transporter catalyzing the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A7 is the primary monocarboxylate transporter in Purkinje cells and can be detected in the brain, cerebellum, liver, and kidney, but not in vascular smooth muscle cells, pericytes, endothelium, heart, lung, or stomach. There are some isoforms of SLC16A7 with bands around 52 kDa and 40-45 kDa in SDS-PAGE. The 80 kDa protein might be the dimer similar to the papers published. (PMID: 9482213, 15749979, 11291733, 16901736)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCT2/SLC16A7 antibody 20355-1-AP | Download protocol |
| IHC protocol for MCT2/SLC16A7 antibody 20355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Dev Cell Lactate shuttling links histone lactylation to adult hippocampal neurogenesis in mice | ||
Br J Cancer Autoregulation of H+/lactate efflux prevents monocarboxylate transport (MCT) inhibitors from reducing glycolytic lactic acid production. | ||
Anim Cells Syst (Seoul) MCT2 overexpression promotes recovery of cognitive function by increasing mitochondrial biogenesis in a rat model of stroke. | ||
J Exp Clin Cancer Res ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer. | ||
Biomed Pharmacother Fibroblast growth factor 21 protects the liver from apoptosis in a type 1 diabetes mouse model via regulating L-lactate homeostasis |
