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SLC16A12 Polyclonal antibody

SLC16A12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20553-1-AP

Print datasheet

Synonyms

CJMG, MCT 12, MCT12, Monocarboxylate transporter 12, SLC16A12

验证数据展示

  • IHC staining of human stomach cancer using 20553-1-AP

    IHC staining of human stomach cancer using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC16A12 antibody included
  • WB analysis of mouse kidney using 20553-1-AP

    WB analysis of mouse kidney using 20553-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20553-1-AP

    IHC staining of human kidney using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC16A12 antibody included
  • IHC staining of mouse testis using 20553-1-AP

    IHC staining of mouse testis using 20553-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC16A12 antibody included

经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman stomach cancer tissue, mouse testis tissue, human kidney tissue, human testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SLC16A12 Ready-To-Use IHC Kit. SLC16A12 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20553-1-AP targets SLC16A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, Xenopus
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 16, member 12 (monocarboxylic acid transporter 12)
Synonyms CJMG, MCT 12, MCT12, Monocarboxylate transporter 12, SLC16A12
Calculated Molecular Weight 53 kDa
Observed Molecular Weight53-56 kDa
GenBank Accession NumberNM_213606
Gene Symbol SLC16A12
Gene ID (NCBI) 387700
RRIDAB_10693621
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZSM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC16A12, also named as MCT12 and CJMG, belongs to the major facilitator superfamily and Monocarboxylate porter (TC 2.A.1.13) family. It is a proton-linked monocarboxylate transporter. SLC16A12 catalyzes the rapid transport across the plasma membrane of many monocarboxylates. The antibody is specific to SLC16A12.

实验方案

Product Specific Protocols
WB protocol for SLC16A12 antibody 20553-1-APDownload protocol
IHC protocol for SLC16A12 antibody 20553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
XenopusWB

Biochem Biophys Res Commun

Molecular characterization of the orphan transporter SLC16A9, an extracellular pH- and Na+-sensitive creatine transporter.

Authors - Yuya Futagi
View Article
humanIHC

Medicine (Baltimore)

Decreased expression of SLC16A12 mRNA predicts poor prognosis of patients with clear cell renal cell carcinoma.

Authors - Jie Mei
View Article