验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human stomach cancer tissue, human kidney tissue, human testis tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SLC16A12 Ready-To-Use IHC Kit. SLC16A12 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
20553-1-AP targets SLC16A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, xenopus |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 16, member 12 (monocarboxylic acid transporter 12) |
| 别名 | CJMG, Creatine transporter 2, CRT2, MCT 12, MCT12 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 53-56 kDa |
| GenBank蛋白编号 | NM_213606 |
| 基因名称 | SLC16A12 |
| Gene ID (NCBI) | 387700 |
| RRID | AB_10693621 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6ZSM3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC16A12, also named as MCT12 and CJMG, belongs to the major facilitator superfamily and Monocarboxylate porter (TC 2.A.1.13) family. It is a proton-linked monocarboxylate transporter. SLC16A12 catalyzes the rapid transport across the plasma membrane of many monocarboxylates. The antibody is specific to SLC16A12.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC16A12 antibody 20553-1-AP | Download protocol |
| WB protocol for SLC16A12 antibody 20553-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Molecular characterization of the orphan transporter SLC16A9, an extracellular pH- and Na+-sensitive creatine transporter. | ||
Medicine (Baltimore) Decreased expression of SLC16A12 mRNA predicts poor prognosis of patients with clear cell renal cell carcinoma. |
