验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy NKCC2/SLC12A1 Ready-To-Use IHC Kit. NKCC2/SLC12A1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13636-1-AP targets SLC12A1/NKCC2 in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Ag4564 Product name: Recombinant human SLC12A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC040138 Sequence: MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 12 (sodium/potassium/chloride transporters), member 1 |
| 别名 | NKCC2, SLC12A1, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter |
| 计算分子量 | 121 kDa |
| 观测分子量 | 140-180 kDa |
| GenBank蛋白编号 | BC040138 |
| 基因名称 | SLC12A1/NKCC2 |
| Gene ID (NCBI) | 6557 |
| RRID | AB_3085420 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13621 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC12A1/NKCC2 antibody 13636-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
