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验证数据展示

  • WB analysis using 15010-1-AP (same clone as 15010-1-PBS)

    WB analysis using 15010-1-AP (same clone as 15010-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

    WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

    WB result of SKP2 antibody (15010-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

    IP experiment of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

    IP result of anti-SKP2 (IP:15010-1-AP, 4ug; Detection:15010-1-AP 1:300) with HEK-293 cells lysate 2440ug. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)

    IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SKP2 antibody included
  • IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)

    IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SKP2 antibody included
  • IF Staining of HepG2 using 15010-1-AP (same clone as 15010-1-PBS)

    IF Staining of HepG2 using 15010-1-AP (same clone as 15010-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKP2 antibody (15010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SKP2 Polyclonal antibody, PBS Only

SKP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15010-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SKP2 Ready-To-Use IHC Kit. SKP2 primary antibody included.

产品信息

15010-1-PBS targets SKP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6826

Product name: Recombinant human SKP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-344 aa of BC001441

Sequence: MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKEEPDSENIPQELLSNLGHPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSLPDELLLGIFSCLCLPELLKVSGVCKRWYRLASDESLWQTLDLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNTLAKNSNLVRLNLSGCSGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQVAVAHVSETITQLNLSGYRKNLQKSDLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFFQLNYLQHLSLSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S-phase kinase-associated protein 2 (p45)
别名
计算分子量 48 kDa
观测分子量 46-48 kDa
GenBank蛋白编号BC001441
基因名称 SKP2
Gene ID (NCBI) 6502
RRIDAB_2187647
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13309
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).

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