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  • Featured Product
  • KD/KO Validated

SKIV2L Polyclonal antibody

SKIV2L Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11462-1-AP

Print datasheet

Synonyms

170A, DDX13, Helicase like protein, Helicase SKI2W, HLP, SKI2, SKI2W, SKIV2, SKIV2L

验证数据展示

  • WB analysis of HEK-293 using 11462-1-AP

    WB analysis of HEK-293 using 11462-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 11462-1-AP

    IP experiment of HepG2 using 11462-1-AP

    IP result of anti-SKIV2L (IP:11462-1-AP, 4ug; Detection:11462-1-AP 1:600) with HepG2 cells lysate 3200ug.

  • WB analysis using 11462-1-AP

    WB analysis using 11462-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, COLO 320 cells, human kidney tissue, HepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11462-1-AP targets SKIV2L in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SKIV2L fusion protein Ag1977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name superkiller viralicidic activity 2-like (S. cerevisiae)
Synonyms 170A, DDX13, Helicase like protein, Helicase SKI2W, HLP, SKI2, SKI2W, SKIV2, SKIV2L
Calculated Molecular Weight 1246 aa, 138 kDa
Observed Molecular Weight 138 kDa
GenBank Accession NumberBC015758
Gene Symbol SKIV2L
Gene ID (NCBI) 6499
RRIDAB_2187472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15477
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

实验方案

Product Specific Protocols
WB protocol for SKIV2L antibody 11462-1-APDownload protocol
IP protocol for SKIV2L antibody 11462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nat Immunol

The SKIV2L RNA exosome limits activation of the RIG-I-like receptors.

Authors - Sterling C Eckard
View Article
WB

Sci Immunol

The mammalian SKIV2L RNA exosome is essential for early B cell development.

Authors - Kun Yang
View Article
humanWB

J Clin Invest

Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.

Authors - Kun Yang
View Article
humanWB

Nucleic Acids Res

Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.

Authors - Takuto Nogimori
  • KD Validated
View Article
humanWB

Nucleic Acids Res

A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.

Authors - Katarzyna Kalisiak
View Article
humanWB

Proc Natl Acad Sci U S A

The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.

Authors - Stuart Weston
  • KD Validated
View Article