验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, COLO 320 cells, human kidney tissue, HepG2 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11462-1-AP targets SKIV2L in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1977 Product name: Recombinant human SKIV2L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 861-1246 aa of BC015758 Sequence: QVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPEGPCDHTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLAQAHPAGPPTLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQYLKLRERMQIQKEMERLRFLLSDQSLLLLPEYHQRVEVLRTLGYVDEVGTVKLAGRVACAMSSHELLLTELMFDNALSTLRPEEIAALLSGLVCQSPGDAGDQLPNTLKQGIERVRAVAKRIGEVQVACGLNQTVEEFVGELNFGLVEVVYEWARGMPFSELAGLSGTPEGLVVRCIQRLAEMCRSLRGAARLVGEPVLGAKMETAATLLRRDIVFAASLYTQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | superkiller viralicidic activity 2-like (S. cerevisiae) |
| 别名 | SKI2, HLP, Helicase SKI2W, EC:3.6.4.13, DDX13 |
| 计算分子量 | 1246 aa, 138 kDa |
| 观测分子量 | 138 kDa |
| GenBank蛋白编号 | BC015758 |
| 基因名称 | SKIV2L |
| Gene ID (NCBI) | 6499 |
| RRID | AB_2187472 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15477 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SKIV2L antibody 11462-1-AP | Download protocol |
| IP protocol for SKIV2L antibody 11462-1-AP | Download protocol |
| WB protocol for SKIV2L antibody 11462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease. | ||
Nucleic Acids Res Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.
| ||
Nucleic Acids Res A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans. | ||
Proc Natl Acad Sci U S A The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.
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