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验证数据展示

  • WB analysis using 21305-1-AP (same clone as 21305-1-PBS)

    WB analysis using 21305-1-AP (same clone as 21305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 21305-1-AP (same clone as 21305-1-PBS)

    IHC staining of human cervical cancer using 21305-1-AP (same clone as 21305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIX4 antibody included
  • IHC staining of human ovary cancer using 21305-1-AP (same clone as 21305-1-PBS)

    IHC staining of human ovary cancer using 21305-1-AP (same clone as 21305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIX4 antibody included
  • IF Staining of HEK-293 using 21305-1-AP (same clone as 21305-1-PBS)

    IF Staining of HEK-293 using 21305-1-AP (same clone as 21305-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIX4 antibody (21305-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

SIX4 Polyclonal antibody, PBS Only

SIX4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21305-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AREC3, Homeobox protein SIX4, SIX homeobox 4

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SIX4 Ready-To-Use IHC Kit. SIX4 primary antibody included.

产品信息

21305-1-PBS targets SIX4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15918

Product name: Recombinant human SIX4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 411-760 aa of BC098282

Sequence: TTTSYSPSVPVSFPGLIPSTEVKREGIQTVASQDGGSVVTFTTPVQINQYGIVQIPNSGANSQFLNGSIGFSPLQLPPVSVAASQGNISVSSSTSDGSTFTSESTTVQQGKVFLSSLAPSAVVYTVPNTGQTIGSVKQEGLERSLVFSQLMPVNQNAQVNANLSSENISGSGLHPLASSLVNVSPTHNFSLSPSTLLNPTELNRDIADSQPMSAPVASKSTVTSVSNTNYATLQNCSLITGQDLLSVPMTQAALGEIVPTAEDQVGHPSPAVHQDFVQEHRLVLQSVANMKENFLSNSESKATSSLMMLDSKSKYVLDGMVDTVCEDLETDKKELAKLQTVQLDEDMQDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SIX homeobox 4
别名 AREC3, Homeobox protein SIX4, SIX homeobox 4
计算分子量 781 aa, 83 kDa
观测分子量 98 kDa
GenBank蛋白编号BC098282
基因名称 SIX4
Gene ID (NCBI) 51804
RRIDAB_10860258
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIU6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Six (sine oculis) proteins are a family of homeodomain transcription factors that share a conserved DNA-binding domain and are human homologs of the Drosophila sine oculis (so) protein. SIX homeobox 4 (SIX4), also known as AREC3, belongs to the the Six family. Six4 contains one Six domain (which funtions as a homeobox DNA-binding motif) and shares 90% sequence similarity with its mouse counterpart, suggesting that both proteins have similar DNA-binding properties. SIX4 is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles.

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