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验证数据展示

  • WB analysis using 84805-4-RR (same clone as 84805-4-PBS)

    WB analysis using 84805-4-RR (same clone as 84805-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84805-4-RR (SIRT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

    IHC staining of rat liver using 84805-4-RR (same clone as 84805-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84805-4-RR (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIRT7 antibody included
  • IP experiment of HEK-293T using 84805-4-RR (same clone as 84805-4-PBS)

    IP experiment of HEK-293T using 84805-4-RR (same clone as 84805-4-PBS)

    IP result of anti-SIRT7 (IP:84805-4-RR, 4ug; Detection:84805-4-RR 1:2000) with HEK-293T cells lysate 2200 ug. This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84805-4-RR (same clone as 84805-4-PBS)

    IF Staining of MCF-7 using 84805-4-RR (same clone as 84805-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SIRT7 antibody (84805-4-RR, Clone: 242167C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84805-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84805-4-RR

    Affinity and Kinetic Characterization of 84805-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84805-4-RR against Human SIRT7 were performed. The affinity constant is below 1 pM.

SIRT7 Recombinant monoclonal antibody, PBS Only

SIRT7 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 84805-4-PBS

产品说明书

CloneNo. 242167C9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

242167C9, EC:2.3.1.-, EC:2.3.1.286, NAD-dependent protein deacylase sirtuin-7, SIR2 like protein 7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SIRT7 Ready-To-Use IHC Kit. SIRT7 primary antibody included.

产品信息

84805-4-PBS targets SIRT7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag31794

Product name: Recombinant human SIRT7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of NM_016538

Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
别名 242167C9, EC:2.3.1.-, EC:2.3.1.286, NAD-dependent protein deacylase sirtuin-7, SIR2 like protein 7
计算分子量45KD
观测分子量 45 kDa
GenBank蛋白编号NM_016538
基因名称 SIRT7
Gene ID (NCBI) 51547
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NRC8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

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