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验证数据展示

  • WB analysis using 84805-4-RR

    WB analysis using 84805-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84805-4-RR (SIRT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 84805-4-RR

    IHC staining of rat liver using 84805-4-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84805-4-RR (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SIRT7 antibody included
  • IP experiment of HEK-293T using 84805-4-RR

    IP experiment of HEK-293T using 84805-4-RR

    IP result of anti-SIRT7 (IP:84805-4-RR, 4ug; Detection:84805-4-RR 1:2000) with HEK-293T cells lysate 2200 ug.

  • Affinity and Kinetic Characterization of 84805-4-RR

    Affinity and Kinetic Characterization of 84805-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84805-4-RR against Human SIRT7 were performed. The affinity constant is below 1 pM.

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SIRT7 Recombinant monoclonal antibody

SIRT7 Uni-rAb® Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 84805-4-RR

产品说明书

CloneNo. 242167C9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IP, ELISA

别名

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HCT 116 cells, Caco-2 cells, HEK-293T cells, rat liver tissue
Positive IP detected inHEK-293T cells
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SIRT7 Ready-To-Use IHC Kit. SIRT7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84805-4-RR targets SIRT7 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag31794

Product name: Recombinant human SIRT7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of NM_016538

Sequence: MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
别名 SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-
计算分子量45KD
观测分子量 45 kDa
GenBank蛋白编号NM_016538
基因名称 SIRT7
Gene ID (NCBI) 51547
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NRC8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

实验方案

Product Specific Protocols
WB protocol for SIRT7 antibody 84805-4-RRDownload protocol
IHC protocol for SIRT7 antibody 84805-4-RRDownload protocol
IP protocol for SIRT7 antibody 84805-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols