SIRP Alpha Polyclonal antibody
SIRP Alpha Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, THP-1 cells, PC-12 cells, U-937 cells, mouse cerebellum tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
14482-1-AP targets SIRP Alpha in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SIRP Alpha fusion protein Ag5865 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | signal-regulatory protein alpha |
| Synonyms | BIT, CD172A, Inhibitory receptor SHPS 1, Macrophage fusion receptor, MFR, MYD 1, MyD 1 antigen, MYD1, P84, PTPNS1, SHP substrate 1, SHPS 1, SHPS1, SIRP, SIRP alpha, SIRP ALPHA 1, Sirp alpha 2, Sirp alpha 3, SIRPA, SIRPalpha, SIRPalpha2 |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | BC033092 |
| Gene Symbol | SIRPA |
| Gene ID (NCBI) | 140885 |
| RRID | AB_2188056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78324 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRP Alpha antibody 14482-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Prolif Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling | ||
Acta Biochim Biophys Sin (Shanghai) Clostridium butyricum regulates visceral hypersensitivity of irritable bowel syndrome by inhibiting colonic mucous low grade inflammation through its action on NLRP6. | ||
J Obstet Gynaecol Res Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages. | ||
Biomedicines Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis |
