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验证数据展示

  • FC experiment of human PBMCs using FITC-98101

    FC experiment of human PBMCs using FITC-98101

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human SIRP Alpha/CD172a Rabbit RecAb (FITC-98101, Clone: 240789E1) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using FITC-98101

    FC experiment of human PBMCs using FITC-98101

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human SIRP Alpha/CD172a Rabbit RecAb (FITC-98101, Clone: 240789E1) (red) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

FITC Plus Anti-Human SIRP Alpha/CD172a Rabbit Recombinant Antibody

SIRP Alpha/CD172a Uni-rAb® Recombinant Antibody for FC
Cat No. FITC-98101

产品说明书

CloneNo. 240789E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SIRP Alpha, SIRPA, 240789E1, BIT, Brain Ig-like molecule with tyrosine-based activation motifs

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  FITC Plus
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-98101 targets SIRP Alpha/CD172a in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg0625

Product name: Recombinant Human SIRP alpha/CD172a protein (His Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 31-370 aa of NM_080792

Sequence: EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal-regulatory protein alpha
别名 SIRP Alpha, SIRPA, 240789E1, BIT, Brain Ig-like molecule with tyrosine-based activation motifs
计算分子量55 kDa
GenBank蛋白编号NM_080792
基因名称 SIRP Alpha
Gene ID (NCBI) 140885
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
激发激光Blue laser (488 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78324
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

实验方案

Product Specific Protocols
FC protocol for FITC Plus SIRP Alpha/CD172a antibody FITC-98101Download protocol
Standard Protocols
Click here to view our Standard Protocols
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