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验证数据展示

  • WB analysis using 30544-1-AP (same clone as 30544-1-PBS)

    WB analysis using 30544-1-AP (same clone as 30544-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30544-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 30544-1-AP (same clone as 30544-1-PBS)

    IF Staining of HeLa using 30544-1-AP (same clone as 30544-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30544-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 30544-1-AP (same clone as 30544-1-PBS)

    IP experiment of HeLa using 30544-1-AP (same clone as 30544-1-PBS)

    IP result of anti-SIPA1L3 (IP:30544-1-AP, 4ug; Detection:30544-1-AP 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 30544-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 30544-1-AP (same clone as 30544-1-PBS)

    IF Staining of HEK-293 using 30544-1-AP (same clone as 30544-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30544-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 30544-1-AP (same clone as 30544-1-PBS)

    IHC staining of human ovary cancer using 30544-1-AP (same clone as 30544-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30544-1-AP (SIPA1L3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30544-1-PBS in a different storage buffer formulation.

SIPA1L3 Polyclonal antibody, PBS Only

SIPA1L3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 30544-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

SPA-1-like protein 3, SPA 1 like protein 3, SIPA1-like protein 3, SIPA1 like protein 3, Signal-induced proliferation-associated 1-like protein 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30544-1-PBS targets SIPA1L3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30750

Product name: Recombinant human SIPA1L3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC150620

Sequence: MTTYRAIPSDGVDLAASCGARVGDVLPGPHTGDYAPLGFWAQNGSMSQPLGESPATATATATATTRPSPTTPAMPKMGVRARVADWPPKREALREHSNPSPSQDTDGTKATKMAHSMRSIQNGQPPTSTPASSGSKAFHRLSRRRSKDVEFQDGWPRSPGRAFLPLRHRSSSEITLSECDAEDAGEPRGARHTGALPLFREYGSTSSIDVQGMPEQSFFDILNEFRSEQPDARGCQALTELLRADPGPHLMGGGGGAKGDSHNGQPAKDSLLPLQPTKEKEKARKKPARGLGGGDTVDSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal-induced proliferation-associated 1 like 3
别名 SPA-1-like protein 3, SPA 1 like protein 3, SIPA1-like protein 3, SIPA1 like protein 3, Signal-induced proliferation-associated 1-like protein 3
观测分子量 195 kDa
GenBank蛋白编号BC150620
基因名称 SIPA1L3
Gene ID (NCBI) 23094
RRIDAB_3086357
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60292
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STEAP3 (Signal-induced proliferation-associated 1-like protein 3) is also named as KIAA0545 and SPAL3. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

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