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SIPA1L3 Polyclonal antibody

SIPA1L3 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 30544-1-AP

Print datasheet

Synonyms

KIAA0545, SIPA1 like protein 3, SIPA1L3, SPA 1 like protein 3, SPAL3

验证数据展示

  • WB analysis using 30544-1-AP

    WB analysis using 30544-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 30544-1-AP

    IF Staining of HeLa using 30544-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 30544-1-AP

    IP experiment of HeLa using 30544-1-AP

    IP result of anti-SIPA1L3 (IP:30544-1-AP, 4ug; Detection:30544-1-AP 1:3000) with HeLa cells lysate 1320 ug.

  • IF Staining of HEK-293 using 30544-1-AP

    IF Staining of HEK-293 using 30544-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30544-1-AP targets SIPA1L3 in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen SIPA1L3 fusion protein Ag30750 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal-induced proliferation-associated 1 like 3
Synonyms KIAA0545, SIPA1 like protein 3, SIPA1L3, SPA 1 like protein 3, SPAL3
Observed Molecular Weight 195 kDa
GenBank Accession NumberBC150620
Gene Symbol SIPA1L3
Gene ID (NCBI) 23094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60292
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP3 (Signal-induced proliferation-associated 1-like protein 3) is also named as KIAA0545 and SPAL3. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

实验方案

Product Specific Protocols
WB protocol for SIPA1L3 antibody 30544-1-APDownload protocol
IF protocol for SIPA1L3 antibody 30544-1-APDownload protocol
IP protocol for SIPA1L3 antibody 30544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols