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SIPA1L1 Polyclonal antibody

SIPA1L1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25086-1-AP

Print datasheet

Synonyms

E6 targeted protein 1, E6TP1, KIAA0440, SIPA1 like protein 1, SIPA1L1

验证数据展示

  • WB analysis using 25086-1-AP

    WB analysis using 25086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25086-1-AP (SIPA1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25086-1-AP

    IHC staining of human brain using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25086-1-AP

    IHC staining of human brain using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 25086-1-AP

    IP experiment of mouse brain using 25086-1-AP

    IP result of anti-SIPA1L1 (IP:25086-1-AP, 4ug; Detection:25086-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25086-1-AP targets SIPA1L1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen SIPA1L1 fusion protein Ag18900 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal-induced proliferation-associated 1 like 1
Synonyms E6 targeted protein 1, E6TP1, KIAA0440, SIPA1 like protein 1, SIPA1L1
Calculated Molecular Weight 1803 aa, 200 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC146777
Gene Symbol SIPA1L1
Gene ID (NCBI) 26037
RRIDAB_2714023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIPA1L1 antibody 25086-1-APDownload protocol
IHC protocol for SIPA1L1 antibody 25086-1-APDownload protocol
IP protocol for SIPA1L1 antibody 25086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Neuron

Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward.

Authors - Sophie Laguesse
View Article