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验证数据展示

  • WB analysis using 85664-4-RR

    WB analysis using 85664-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85664-4-RR (SII-TFIIS,TCEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85664-4-RR

    IF Staining of HeLa using 85664-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 85664-4-RR

    IF Staining of A431 using 85664-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SII-TFIIS,TCEA1 Recombinant antibody

SII-TFIIS,TCEA1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85664-4-RR

产品说明书

CloneNo. 243155H8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

TCEA1, GTF2S, SII, SII TFIIS, TCEA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, SGC-7901 cells, K-562 cells, Sp2/0 cells
Positive IF/ICC detected inA431 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy TCEA1 Ready-To-Use IHC Kit. TCEA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85664-4-RR targets SII-TFIIS,TCEA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SII-TFIIS,TCEA1 fusion protein Ag12199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription elongation factor A (SII), 1
别名 TCEA1, GTF2S, SII, SII TFIIS, TCEA
计算分子量 301 aa, 34 kDa
观测分子量40 kDa
GenBank蛋白编号BC072460
基因名称 TCEA1
Gene ID (NCBI) 6917
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23193
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.

实验方案

Product Specific Protocols
WB protocol for SII-TFIIS,TCEA1 antibody 85664-4-RRDownload protocol
IF protocol for SII-TFIIS,TCEA1 antibody 85664-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols