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验证数据展示

  • WB analysis using 85664-4-RR (same clone as 85664-4-PBS)

    WB analysis using 85664-4-RR (same clone as 85664-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85664-4-RR (SII-TFIIS,TCEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85664-4-RR (same clone as 85664-4-PBS)

    IF Staining of HeLa using 85664-4-RR (same clone as 85664-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 85664-4-RR (same clone as 85664-4-PBS)

    IF Staining of A431 using 85664-4-RR (same clone as 85664-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

SII-TFIIS,TCEA1 Recombinant monoclonal antibody, PBS Only

SII-TFIIS,TCEA1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85664-4-PBS

产品说明书

CloneNo. 243155H8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

TCEA1, GTF2S, SII, SII TFIIS, TCEA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TCEA1 Ready-To-Use IHC Kit. TCEA1 primary antibody included.

产品信息

85664-4-PBS targets SII-TFIIS,TCEA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12199

Product name: Recombinant human SII-TFIIS,TCEA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC072460

Sequence: MEDEVVRFAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQSTRIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPEAREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription elongation factor A (SII), 1
别名 TCEA1, GTF2S, SII, SII TFIIS, TCEA
计算分子量 301 aa, 34 kDa
观测分子量40 kDa
GenBank蛋白编号BC072460
基因名称 TCEA1
Gene ID (NCBI) 6917
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23193
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.

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