Siglec-7 Polyclonal antibody
Siglec-7 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | U-937 cells, A549 cells, human liver tissue, human placenta tissue, K-562 cells, L02 cells |
Positive IHC detected in | human tonsillitis tissue, human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | U-937 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13939-1-AP targets Siglec-7 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | Siglec-7 fusion protein Ag4220 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | sialic acid binding Ig-like lectin 7 |
Synonyms | AIRM 1, AIRM1, CD328, CDw328, D siglec, p75, p75/AIRM1, QA79, QA79 membrane protein, SIGLEC 7, SIGLEC7, Siglec-7 |
Calculated Molecular Weight | 467 aa, 51 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC028150 |
Gene Symbol | SIGLEC7 |
Gene ID (NCBI) | 27036 |
RRID | AB_10919158 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y286 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Siglec-7 antibody 13939-1-AP | Download protocol |
IHC protocol for Siglec-7 antibody 13939-1-AP | Download protocol |
FC protocol for Siglec-7 antibody 13939-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncol Lett Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer. | ||
Commun Biol ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer |