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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83389-4-RR (same clone as 83389-4-PBS)

    WB analysis using 83389-4-RR (same clone as 83389-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83389-4-RR (SIAH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

  • WB analysis of mouse kidney using 83389-4-RR (same clone as 83389-4-PBS)

    WB analysis of mouse kidney using 83389-4-RR (same clone as 83389-4-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 83389-4-RR (SIAH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83389-4-RR (same clone as 83389-4-PBS)

    IHC staining of mouse testis using 83389-4-RR (same clone as 83389-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83389-4-RR (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIAH1 antibody included
  • FC experiment of A549 using 83389-4-RR (same clone as 83389-4-PBS)

    FC experiment of A549 using 83389-4-RR (same clone as 83389-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Siah1 Recombinant Antibody (83389-4-RR, Clone:240377B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83389-4-RR

    Affinity and Kinetic Characterization of 83389-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83389-4-RR against Human SIAH1 were performed. The affinity constant is 2.39 nM.

SIAH1 Recombinant antibody, PBS Only

SIAH1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83389-4-PBS

产品说明书

CloneNo. 240377B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Siah-1a, Siah-1, RING-type E3 ubiquitin transferase SIAH1, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SIAH1 Ready-To-Use IHC Kit. SIAH1 primary antibody included.

产品信息

83389-4-PBS targets SIAH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SIAH1 fusion protein Ag34618 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 seven in absentia homolog 1 (Drosophila)
别名 Siah-1a, Siah-1, RING-type E3 ubiquitin transferase SIAH1, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH1
计算分子量 282 aa, 31 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC035562
基因名称 SIAH1
Gene ID (NCBI) 6477
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8IUQ4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)