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验证数据展示

  • WB analysis using 19900-1-AP

    WB analysis using 19900-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19900-1-AP (SI-CLP,CHID1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SI-CLP,CHID1 Polyclonal antibody

SI-CLP,CHID1 Polyclonal Antibody for WB, ELISA
Cat No. 19900-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CHID1, SB139, PSEC0104, GL008, Chitinase domain-containing protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19900-1-AP targets SI-CLP,CHID1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13806

Product name: Recombinant human SI-CLP,CHID1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 19-393 aa of BC000001

Sequence: TTLSKSDAKKAASKTLLEKSQFSDKPVQDRGLVVTDLKAESVVLEHRSYCSAKARDRHFAGDVLGYVTPWNSHGYDVTKVFGSKFTQISPVWLQLKRRGREMFEVTGLHDVDQGWMRAVRKHAKGLHIVPRLLFEDWTYDDFRNVLDSEDEIEELSKTVVQVAKNQHFDGFVVEVWNQLLSQKRVGLIHMLTHLAEALHQARLLALLVIPPAITPGTDQLGMFTHKEFEQLAPVLDGFSLMTYDYSTAHQPGPNAPLSWVRACVQVLDPKSKWRSKILLGLNFYGMDYATSKDAREPVVGARYIQTLKDHRPRMVWDSQASEHFFEYKKSRSGRHVVFYPTLKSLQVRLELARELGVGVSIWELGQGLDYFYDLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chitinase domain containing 1
别名 CHID1, SB139, PSEC0104, GL008, Chitinase domain-containing protein 1
计算分子量 393 aa, 45 kDa
观测分子量42-48 kDa
GenBank蛋白编号BC000001
基因名称 CHID1
Gene ID (NCBI) 66005
RRIDAB_3085591
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BWS9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHID1 (chitinase domain containing protein 1) is a member of the Glyco_18 domain-containing proteins family, expressed by type 2 macrophages upon their stimulation with interleukin (IL)-4 and glucocorticoids. Expression of CHID1 is documented for TAMs in various tumors, but its role in tumor development remains unclear. (PMID: 33466316)

实验方案

Product Specific Protocols
WB protocol for SI-CLP,CHID1 antibody 19900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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