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SHQ1 Polyclonal antibody

SHQ1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 27020-1-AP

Print datasheet

Synonyms

Protein SHQ1 homolog, SHQ1, SHQ1 homolog (S. cerevisiae)

验证数据展示

  • WB analysis using 27020-1-AP

    WB analysis using 27020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27020-1-AP (SHQ1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27020-1-AP

    IHC staining of human colon cancer using 27020-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27020-1-AP (SHQ1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27020-1-AP

    IHC staining of human colon cancer using 27020-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27020-1-AP (SHQ1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 27020-1-AP

    IF Staining of A431 using 27020-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 27020-1-AP (SHQ1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, A549 cells, HEK-293T cells
Positive IHC detected inhuman colon cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27020-1-AP is SHQ1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IP, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen SHQ1 fusion protein Ag25559 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SHQ1 homolog (S. cerevisiae)
Synonyms Protein SHQ1 homolog, SHQ1, SHQ1 homolog (S. cerevisiae)
Calculated Molecular Weight 593 aa, 67 kDa
Observed Molecular Weight 70 kDa~80 kDa
GenBank Accession NumberBC017274
Gene Symbol SHQ1
Gene ID (NCBI) 55164
RRIDAB_2880725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PI26
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHQ1 antibody 27020-1-APDownload protocol
IHC protocol for SHQ1 antibody 27020-1-APDownload protocol
IF protocol for SHQ1 antibody 27020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nucleic Acids Res

Proteomic analyses reveal new features of the box H/ACA RNP biogenesis

Authors - Florence Schlotter
View Article
humanWB,IP,CoIP

Hum Mol Genet

The C-terminal extension of dyskerin is a dyskeratosis congenita mutational hotspot that modulates interaction with telomerase RNA and subcellular localization

Authors - Jian Qin
  • KO Validated
View Article