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验证数据展示

  • WB analysis using 27020-1-AP

    WB analysis using 27020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27020-1-AP (SHQ1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27020-1-AP

    IHC staining of human colon cancer using 27020-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27020-1-AP (SHQ1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27020-1-AP

    IHC staining of human colon cancer using 27020-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27020-1-AP (SHQ1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 27020-1-AP

    IF Staining of A431 using 27020-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 27020-1-AP (SHQ1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of K-562 using 27020-1-AP

    IP experiment of K-562 using 27020-1-AP

    IP result of anti-SHQ1 (IP:27020-1-AP, 4ug; Detection:27020-1-AP 1:1500) with K-562 cells lysate 2400 ug.

SHQ1 Polyclonal antibody

SHQ1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 27020-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

Protein SHQ1 homolog, SHQ1, SHQ1 homolog (S. cerevisiae)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, A549 cells, HEK-293T cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27020-1-AP targets SHQ1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IP, CoIP
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag25559

Product name: Recombinant human SHQ1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-214 aa of BC017274

Sequence: MLTPAFDLSQDPDFLTIAIRVPYARVSEFDVYFEGSDFKFYAKPYFLRLTLPGRIVENGSEQGSYDADKGIFTIRLPKETPGQHFEGLNMLTALLAPRKSRTAKPLVEEIGASEIPEEVVDDEEFDWEIEQTPCEEVSESALNPQCHYGFGNLRSGVLQRLQDELSDVIDIKDPDFTPAAERRQKRLAAELAKFDPDHYLADFFEDEAIEQILK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SHQ1 homolog (S. cerevisiae)
别名 Protein SHQ1 homolog, SHQ1, SHQ1 homolog (S. cerevisiae)
计算分子量 593 aa, 67 kDa
观测分子量 70 kDa~80 kDa
GenBank蛋白编号BC017274
基因名称 SHQ1
Gene ID (NCBI) 55164
RRIDAB_2880725
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PI26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHQ1 antibody 27020-1-APDownload protocol
IHC protocol for SHQ1 antibody 27020-1-APDownload protocol
IF protocol for SHQ1 antibody 27020-1-APDownload protocol
IP protocol for SHQ1 antibody 27020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nucleic Acids Res

Proteomic analyses reveal new features of the box H/ACA RNP biogenesis

Authors - Florence Schlotter
View Article
humanWB,IP,CoIP

Hum Mol Genet

The C-terminal extension of dyskerin is a dyskeratosis congenita mutational hotspot that modulates interaction with telomerase RNA and subcellular localization

Authors - Jian Qin
View Article
human,mouseWB

Exp Neurol

Pathogenic SHQ1 variants result in disruptions to neuronal development and the dopaminergic pathway

Authors - Chien-Hui Chang
  • KD Validated
View Article