验证数据展示
产品信息
85758-5-PBS targets SHMT1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), Indirect ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag31269 Product name: Recombinant human SHMT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 320-483 aa of BC038598 Sequence: MTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | serine hydroxymethyltransferase 1 (soluble) |
| 别名 | CSHMT, EC:2.1.2.1 |
| 计算分子量 | 483 aa, 53 kDa |
| 观测分子量 | 45-53 kDa |
| GenBank蛋白编号 | BC038598 |
| 基因名称 | SHMT1 |
| Gene ID (NCBI) | 6470 |
| RRID | AB_3744353 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P34896 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
SHMT1 (serine hydroxymethyltransferase 1) is a cytosolic enzyme that plays a central role in one-carbon metabolism, catalyzing the reversible conversion of serine and tetrahydrofolate (THF) to glycine and 5,10-methylenetetrahydrofolate (5,10-methylene-THF). This reaction provides the primary source of one-carbon units for a variety of essential biosynthetic processes, including the synthesis of purines, thymidylate, and methionine, as well as for methylation reactions and redox balance maintenance. SHMT1 is highly expressed in proliferating tissues and cancer cells, where its activity supports increased nucleotide synthesis and cell growth. Additionally, SHMT1 is regulated at both transcriptional and post-translational levels, and its dysregulation has been linked to metabolic disorders, developmental defects, and cancer progression. The enzyme's critical role in compartmentalized one-carbon metabolism makes it an important target for therapeutic intervention, particularly in oncology.
