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验证数据展示

  • WB analysis using 29752-1-AP

    WB analysis using 29752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 29752-1-AP

    IHC staining of mouse embryo using 29752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SHH antibody included
  • IHC staining of mouse embryo using 29752-1-AP

    IHC staining of mouse embryo using 29752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SHH antibody included
  • WB analysis using 29752-1-AP

    WB analysis using 29752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 29752-1-AP

    FC experiment of HeLa using 29752-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SHH (29752-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

SHH Polyclonal antibody

SHH Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 29752-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

EC:3.1.-.-, HHG 1, HHG1, HHG-1, HLP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SHH Ready-To-Use IHC Kit. SHH primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29752-1-AP targets SHH in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31338

Product name: Recombinant human SHH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-123 aa of NM_000193

Sequence: CGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sonic hedgehog homolog (Drosophila)
别名 EC:3.1.-.-, HHG 1, HHG1, HHG-1, HLP3
计算分子量 50 kDa
观测分子量50-60 kDa
GenBank蛋白编号NM_000193
基因名称 SHH
Gene ID (NCBI) 6469
RRIDAB_2935475
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15465
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHH, Sonic hedgehog protein, binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PMID: 10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PMID: 10753901). SHH is synthesized as a 45 kDa precursor that undergoes an autocatalytic processing event that produces a 19 kDa N-terminal product, responsible for all signaling activities, and a 25 kDa C-terminal fragment (PMID: 10753901, PMID: 16282375).

实验方案

Product Specific Protocols
FC protocol for SHH antibody 29752-1-APDownload protocol
IHC protocol for SHH antibody 29752-1-APDownload protocol
WB protocol for SHH antibody 29752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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