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验证数据展示

  • WB analysis using 68647-1-Ig

    WB analysis using 68647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68647-1-Ig (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 68647-1-Ig

    IHC staining of human liver cancer using 68647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 68647-1-Ig

    IHC staining of human liver cancer using 68647-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 68647-1-Ig

    IF Staining of HEK-293 using 68647-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SHCBP1 antibody (68647-1-Ig, Clone: 1B2E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

SHCBP1 Monoclonal antibody

SHCBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68647-1-Ig

产品说明书

CloneNo. 1B2E12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SHC SH2 domain-binding protein 1, PAL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68647-1-Ig targets SHCBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17517

Product name: Recombinant human SHCBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 374-672 aa of BC030699

Sequence: NACFEGDTVIVCPGHYVVHGTFSIADSIELEGYGLPDDIVIEKRGKGDTFVDCTGADIKISGIKFVQHDAVEGILIVHRGKTTLENCVLQCETTGVTVRTSAEFLMKNSDLYGAKGAGIEIYPGSQCTLSDNGIHHCKEGILIKDFLDEHYDIPKISMVNNIIHNNEGYGVVLVKPTIFSDLQESAEDGTEENKALKIQTSGEPDVAERVDLEELIECATGKMELCARTDPSEQVEGNCEIVNELIAASTQKGQIKKKRLSELGITQADDNLMSQEMFVGIVGNQFKWNGKGSFGTFLF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SHC SH2-domain binding protein 1
别名 SHC SH2 domain-binding protein 1, PAL
计算分子量 672 aa, 76 kDa
观测分子量76 kDa
GenBank蛋白编号BC030699
基因名称 SHCBP1
Gene ID (NCBI) 79801
RRIDAB_3670390
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8NEM2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.

实验方案

Product Specific Protocols
IF protocol for SHCBP1 antibody 68647-1-IgDownload protocol
IHC protocol for SHCBP1 antibody 68647-1-IgDownload protocol
WB protocol for SHCBP1 antibody 68647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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